It is of great significance to develop natural active substances for treating Alzheimer’s disease (AD). We constructed an AD cell model using Aβ
25−35-induced PC12 cells to assess isolated components from Tibet wild Gymnadenia crassinervis protective and reparative effects against Aβ
25−35-induced cell injury. The results indicated that the active fraction extracted and isolated from Gymnadenia crassinervis strongly inhibited acetylcholinesterase (AChE) with an excellent IC50 value as low as 135.86 ± 5.59 μg/mL. The main compound of the Gymnadenia crassinervis active fraction was preliminarily identified as dactylorhin A by LC-MS. The high-concentration group, treated with 10 μg/mL of the Gymnadenia crassinervis active fraction, exhibited significantly protective effects, the treatment of which enhanced cell viability and mitigated cell shrinking induced by Aβ
25−35. The ROS level and MDA content in the high-concentration group cells decreased by approximately 28% and 30%, respectively, while the activities of SOD and CAT even increased by about 92% and 221%, respectively. In addition, Western blotting analysis showed that the Gymnadenia crassinervis active fraction inhibited apoptosis. Therefore, these findings suggest that the Gymnadenia crassinervis active fraction can repair Aβ
25−35-induced cell injury, protect against Aβ toxicity, and hold potential in both preventive and therapeutic aspects of AD.
Journal of Food Biochemistry, Volume 2024, Issue 1, 2024. Read More
Related posts:
Network Pharmacology Prediction and Experimental Validation of Ferulic Acid’s Protective Effects against Diclofenac‐Induced Liver Injury
Neuroprotective Effect of Fructus Ligustri Lucidi in Spinal Cord Neuron Injury during H2O2‐Mediated Oxidative Stress
Immune‐Enhancing Activity of Vitis coignetiae Extract via Increasing Cytokine and Natural Killer Cell Activity in Splenocytes and Cyclophosphamide‐Induced Immunosuppressed Rats
Beneficial role of Boehmeria nivea in health and phytochemical constituents
Corn Husk Ferulic Acid Induces Apoptosis of Breast Cancer Cells via ROS‐Mediated Signaling Pathways
Effects of Microwave Pretreatment on Licorice Cell Structure, Components Content, and Physical Properties