Auxin is an essential plant hormone for coordination of plant development. Its signaling pathway is well characterised and its establishment was crucial in understanding how plants react to this hormone at the single cell level. The recent discovery of AC activity in the auxin receptor TIR1 adds a new layer to this signaling pathway that could reshape what we thought we knew about this system and reopens the debate about the role of cAMP in plants.
This master’s thesis explores the newly discovered role of cAMP in the auxin signaling pathway through a combination of synthetic and computational biology. The system was studied in vivo through the engineering and characterisation of a synthetic circuit containing proteins from the auxin signaling pathway in yeast. Different mechanisms of action for the newly discovered influence of cAMP were explored through the development of computational ODE models.
The results show the AC activity mutant having a delayed response to auxin suggesting the role of cAMP includes coordinating a fast response. Differences between strains could point to a basal AC activity of the TIR1 receptor. Finally, the computational models hint towards effects on regulatory complex stability as a mechanism for cAMP influence being more likely than direct stimulation of ARF activity.
Auxin is an essential plant hormone for coordination of plant development. Its signaling pathway is well characterised and its establishment was crucial in understanding how plants react to this hormone at the single cell level. The recent discovery of AC activity in the auxin receptor TIR1 adds a new layer to this signaling pathway that could reshape what we thought we knew about this system and reopens the debate about the role of cAMP in plants.
This master’s thesis explores the newly discovered role of cAMP in the auxin signaling pathway through a combination of synthetic and computational biology. The system was studied in vivo through the engineering and characterisation of a synthetic circuit containing proteins from the auxin signaling pathway in yeast. Different mechanisms of action for the newly discovered influence of cAMP were explored through the development of computational ODE models.
The results show the AC activity mutant having a delayed response to auxin suggesting the role of cAMP includes coordinating a fast response. Differences between strains could point to a basal AC activity of the TIR1 receptor. Finally, the computational models hint towards effects on regulatory complex stability as a mechanism for cAMP influence being more likely than direct stimulation of ARF activity. Read More
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